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The Ensembl genome browser at www.ensembl.org allows scientists to investigate biological annotation of chordate (mostly vertebrate) genomes. Protein-coding and non-coding genes, splice variants, conserved regions across species, sequence variation and information about gene regulation are all available. EnsemblGenomes at www.ensemblgenomes.org extends genome browsing to bacteria, metazoa, protists, plants and fungi. BioMart allows all users to quickly export data in formats that can be opening in MS Excel. A Perl API allows direct access to Ensembl databases. All Ensembl resources are free and open source. We make these videos using Camtasia (TechSmith).